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Studies on antigens from agents causing black grain eumycetoma.

H Romero1, D W Mackenzie

  • 1Universidad de Los Andes, Merida, Venezuela.

Journal of Medical and Veterinary Mycology : Bi-Monthly Publication of the International Society for Human and Animal Mycology
|January 1, 1989
PubMed
Summary

Black grain eumycetoma fungal agents were compared using immunodiffusion techniques. Madurella grisea and Pyrenochaeta mackinnonii showed significant antigenic similarity, suggesting they may be identical.

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Area of Science:

  • Medical Mycology
  • Immunology
  • Infectious Diseases

Background:

  • Black grain eumycetoma is a serious fungal infection.
  • Accurate identification of causative agents is crucial for treatment.
  • Previous classifications relied on morphology, which can be unreliable.

Purpose of the Study:

  • To compare the antigenic profiles of various black grain eumycetoma agents.
  • To investigate the antigenic relationships between different fungal species.
  • To aid in the accurate identification and classification of eumycetoma pathogens.

Main Methods:

  • Preparation of culture filtrate and cellular antigens from 14 fungal agents.
  • Double diffusion and immunoelectrophoresis assays were performed.

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  • Antigenic cross-reactivity was quantified as percentage homology after absorption.

    • Main Results:

    • High cross-reactivity (up to 90%) observed within Madurella grisea isolates and between M. grisea and Pyrenochaeta mackinnonii.
    • Limited antigenic similarity between M. grisea and Madurella mycetomatis.
    • Leptosphaeria senegalensis showed partial homology with M. grisea and P. mackinnonii.
    • Five unidentified isolates exhibited strong antigenic similarity to M. grisea and P. mackinnonii.

    Conclusions:

    • Madurella grisea and Pyrenochaeta mackinnonii are antigenically similar, potentially representing the same species.
    • Antigenic analysis provides a valuable tool for differentiating eumycetoma agents.
    • This study aids in clarifying the taxonomy of black grain eumycetoma pathogens.