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Utility of multiple-locus variant-repeat analysis method for the outbreak of the Pseudomonas aeruginosa isolates

Juan Wang, Xiuming Zhang, Geqin Sun

    Clinical Laboratory
    |August 20, 2014
    PubMed
    Abstract

    Insights

    This study used multiple-locus variable-number tandem repeat (MLVA) analysis to investigate Pseudomonas aeruginosa strains. While no transmission was found, a 4-locus MLVA scheme offers robust discrimination for future studies.

    Area of Science:

    • Microbiology
    • Genetics
    • Epidemiology

    Background:

    • Pseudomonas aeruginosa is a common hospital-acquired pathogen.
    • Understanding strain relatedness is crucial for outbreak investigation and control.

    Purpose of the Study:

    • To determine the source of P. aeruginosa strains in a hospital setting.
    • To evaluate the discriminatory power of different multiple-locus variable-number tandem repeat (MLVA) marker sets.
    • To identify an optimal MLVA scheme for P. aeruginosa strain typing.

    Main Methods:

    • Analyzed 97 P. aeruginosa isolates using multiple-locus variable-number tandem repeat (MLVA) analysis.
    • Evaluated various sets of VNTR loci for their ability to discriminate between strains.
    • Utilized phylogenetic clustering and genotypic analysis to group strains.
    • Assessed drug resistance patterns and specific genetic markers (IMP-1, aac(6')-I, OprD2 deletion).

    Main Results:

    • All 11 VNTR loci showed high discrimination, with the ms223 locus having the lowest discrimination (h = 0.59).
    • A 7-locus MLVA set achieved the same high discrimination (HGDI = 1.0000) as the 11-locus set.
    • A 4-locus set (ms212, ms211, ms213, ms142) provided high discrimination (HGDI = 0.9972) and is recommended for a simplified scheme.
    • Four distinct strain groups were identified, showing significant differences in drug resistance profiles.
    • Four strains from the same ward shared identical drug resistance, MLVA genotype, IMP-1 allele, aac(6')-I type, and OprD2 gene deletion.

    Conclusions:

    • No direct transmission of P. aeruginosa strains was identified in this study.
    • A reduced 4-locus MLVA scheme is proposed as a practical and effective tool for P. aeruginosa typing.
    • The genetic similarity among four strains from the same ward suggests a common source or clonal relationship, supported by shared drug resistance and specific genetic markers.

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