[Focus on the biogenesis of hypophyseal gonadotropins]

We have studied the regulation of the biosynthesis of pituitary gonadotropins in the rat by gonadal steroids and a hypothalamic hormone, GnRH. The methodology used for studying the action of steroids, was either cell-free translation of pituitary messenger RNAs, or hybridization (Northern blot) with synthetic oligodeoxynucleotides (ODN), and for studying the effect of GnRH, primary anterior pituitary cell culture. Our results show that gonadectomy increases and injection of gonadal steroids into gonadectomized rats diminishes the rate of synthesis of the gonadotropin subunit precursors. Progesterone acts only after induction of its pituitary receptors in ovariectomized rats with estradiol benzoate. Thyroxine modulates the action of steroids. Hybridization experiments suggest that gonadal steroids act on the expression of genes encoding the precursors of gonadotropin subunits. GnRH significantly increases incorporation of the labeled amino acids into polypeptide chains of both alpha and LH beta subunits. Intracellular mediators of hormone action, such as cyclic AMP and diacylglycerols, mimic the stimulatory action of GnRH on the synthesis of LH subunits. However, we have no evidence that these products intervene in the effect of GnRH on the LH subunit synthesis. In conclusion, the synthesis of LH and FSH subunits is regulated, with opposite effects, by gonadal steroids which exert their negative control at the genomic level and by GnRH which proceeds via different, yet unknown mechanisms.