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Updated: Apr 25, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
Characterization of a novel AmpC β-lactamase produced by a carbapenem-resistant Cedecea davisae clinical isolate
Nacim Ammenouche1, Hervé Dupont1, Hedi Mammeri2
1Service de Réanimation Polyvalente, Centre Hospitalo-Universitaire d'Amiens, Hôpital Nord, Université de Picardie Jules Verne, Amiens, France INSERM U1088, Université de Picardie Jules Verne, Amiens, France.
Abstract:
A Cedecea davisae isolate, which was intermediate or resistant to third-generation cephalosporins and carbapenems, was recovered from a urine sample. Susceptibility testing, isoelectric focusing, and analysis of outer membrane proteins showed that AmpC β-lactamase expression combined with porin deficiency accounted for the carbapenem resistance. A cloning experiment followed by phenotypic and enzymatic characterization identified a novel class C enzyme that was phylogenetically and biochemically close to the chromosome-borne β-lactamases of the genera Enterobacter and Citrobacter.
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