Related Experiment Videos
Cytofluorometric study on lectin binding of isolated guinea pig keratinocytes
H Nomura1, H Hachisuka, M Higuchi
1Department of Dermatology, Kurume University School of Medicine, Japan.
Acta Histochemica
|January 1, 1989
Abstract:
Lectin binding was cytofluorometrically measured on fractionated keratinocytes of guinea pig. Free keratinocytes were obtained by treatment of EDTA and trypsin. After the treatment, they were separated into 3 fractions by centrifugation on a continuous colloidal silica (Percoll) density gradient. Cells in each fraction were stained by biotinyl lectins and avidin-FITC, and fluorescence intensity was measured by cytofluorometry. Results obtained indicate that little cell surface glycoconjugate is lost during the preparation of free keratinocytes.