Related Experiment Video
Updated: Aug 2, 2026
Candida albicans Biofilm Chip CaBChip for High-throughput Antifungal Drug Screening
Published on: July 18, 2012
[Analysis of ocular toxicity of fluconazole and voriconazole eyedrops using HET-CAM]
A Fernández-Ferreiro1, M González Barcia2, M Gil Martínez3
1Servicio de Farmacia. Hospital Clínico Universitario de Santiago. Gerencia de Gestión Integrada de Santiago de Compostela. Departamento de Farmacia y Tecnología Farmacéutica. Universidad de Santiago de Compostela.. anxordes@gmail.com.
Purpose:
The objective of the study is to provide toxicological information through the HET-CAM test of Fluconazole and Voriconazole eye drops prepared commonly in Pharmacy Services for the treatment of fungal keratitis.
Method:
Experimental Study. The ocular toxicity of topical voriconazole 10 mg/ml and fluconazole 2 mg/ml were evaluated by the hen's egg test (HET) on the chorioallantoic membrane (CAM). The effects on blood vessels were based on its behavior during 300 seconds and processes that may occur at each time, then we calculated the irritation index (is, irritation score).
Results And Conclusions:
Both eye drops, voriconazol and fluconazole have been proven to be safe, since the IS that we obtained was zero for both samples and did not present significant signs of irritation. Therefore, these eyedrops are considered suitable for ocular use from a toxicological point of view.
