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Related Experiment Video

Updated: Apr 25, 2026

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

Published on: March 7, 2019

Boronate affinity nanoparticles for nucleoside separation.

Burcu Okutucu¹, Deniz Vurmaz¹, Akile Tuncal¹

¹a Department of Biochemistry , Ege University , İzmir , Turkey.

Artificial Cells, Nanomedicine, and Biotechnology

|August 20, 2014

Summary

This study developed novel phenylboronic acid-functionalized poly(HEMA) nanoparticles for efficient nucleoside adsorption. These advanced nanomaterials offer a valuable tool for the specific separation of nucleosides in various applications.

Keywords:

boronate affinity nanoparticle nucleoside phenylboronic acid

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Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

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Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

Area of Science:

Biochemistry and Materials Science
Nanotechnology and Affinity Chromatography

Background:

Boronate affinity systems are effective for isolating biomolecules with cis-diol groups, including nucleosides.
Nanosized materials offer unique properties and high surface areas, making them suitable for biotechnological applications.

Purpose of the Study:

To develop a novel boronate affinity system specifically for nucleoside adsorption.
To synthesize and characterize poly(HEMA) nanoparticles functionalized with phenylboronic acid.

Main Methods:

Surfactant-free emulsion polymerization was used to synthesize poly(HEMA) nanoparticles.
Nanoparticles were characterized using Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and Zeta size analysis.
Nucleic acid adsorption experiments were optimized by varying pH, nucleoside concentration, temperature, and ionic strength.

Main Results:

Successfully synthesized and characterized phenylboronic acid-functionalized poly(HEMA) nanoparticles.
Determined optimal adsorption conditions for nucleosides based on experimental parameters.
Demonstrated the effectiveness of the developed system for nucleoside separation.

Conclusions:

The developed boronate ligand-carrying nanoparticles are highly valuable for the specific separation of nucleosides.
This system presents a promising advancement in affinity chromatography for nucleoside isolation.