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Updated: Apr 25, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
Published on: November 21, 2023
Miniature continuous-flow polymerase chain reaction: a breakthrough?
1Institut für Biochemie der Ludwig-Maximilians-Universität Genzentrum, Feodor-Lynen-Strasse 25, D-81377 München, Germany, Fax: (+49) 89-74017-448. zorbas@lmb.uni-muenchen.de.
Abstract:
A time-space conversion enables the polymerase chain reaction (PCR) to be carried out in a continuous-flow process: the mobile reaction mixture is pumped continuously through a glass microchip and passes many times through three constant temperature zones (see picture). The flow rate can be varied to obtain an amplification time of only 90 s. When combined with other continuous-flow microdevices this micromachine may prove useful for routine medical applications and biochemical research.
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