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Updated: Apr 25, 2026

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Published on: December 12, 2017
A spreader-bar approach to molecular architecture: formation of stable artificial chemoreceptors
V M Mirsky1, T Hirsch, S A Piletsky
1Institut für Analytische Chemie Chemo- and Biosensorik der Universität, D-93040 Regensburg, Germany, Fax: (+49) 941-943-4064. vladimir.mirsky@chemie.uni-regensburg.de.
Abstract:
The destructive influence of lateral diffusion on nanostructured monolayers can be prevented by using the spreader-bar technique. This approach allows the formation of stable artificial receptors for barbituric acid by lateral structuring of a dodecanethiol monolayer with molecular spreader-bars from thiobarbituric acid without chemical polymerization (see schematic representation). The new technique may have applications in chemosensors, affinity chromatography, stereoselective catalysis, and molecular electronics.
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