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Related Experiment Video

Updated: Apr 25, 2026

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

Characterizing Pluripotent Stem Cells Using the TaqMan® hPSC Scorecard(TM) Panel.

Jeffrey Fergus¹, Rene Quintanilla, Uma Lakshmipathy

¹Life Sciences Solutions, Thermo Fisher Scientific, 5781 Van Allen Way, Carlsbad, CA, 92008, USA.

Methods in Molecular Biology (Clifton, N.J.)

|August 21, 2014

Summary

Characterizing induced pluripotent stem cells (iPSCs) is challenging due to scale. A new TaqMan(®) hPSC Scorecard™ Panel offers rapid, standardized genetic analysis for functional pluripotency confirmation.

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Last Updated: Apr 25, 2026

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Published on: July 30, 2016

Area of Science:

Stem Cell Biology
Molecular Biology
Genomics

Background:

Patient-specific induced pluripotent stem cells (iPSCs) are crucial for drug discovery and regenerative medicine.
Current characterization methods for iPSCs are laborious and not high-throughput.
Standardized, rapid assays are needed for large-scale iPSC analysis.

Purpose of the Study:

To describe a molecular assay for rapid characterization and standardization of pluripotent stem cells.
To introduce the TaqMan(®) hPSC Scorecard™ Panel as a tool for assessing pluripotency.
To provide a high-throughput method for confirming functional pluripotency.

Main Methods:

Utilized the TaqMan(®) hPSC Scorecard™ Panel, a 94-gene expression real-time PCR assay.
Analyzed gene expression data using cloud-based software against a reference standard.
Assessed undifferentiated and differentiated states of ESCs and iPSCs.

Main Results:

The assay successfully confirmed self-renewal signatures in undifferentiated stem cells.
The system determined differentiation potential by analyzing gene expression in differentiated cells.
Expression signatures were used to define and confirm cell states, replacing complex in vivo assays.

Conclusions:

The TaqMan(®) hPSC Scorecard™ Panel provides a rapid and standardized method for confirming functional pluripotency of ESCs and iPSCs.
This genetic analysis tool supports high-throughput environments for stem cell characterization.
The assay offers flexibility in sample input and preparation, enhancing its utility.