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Lethal Alleles

Lethal Alleles

Agouti: A Lethal Allele
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...

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Related Experiment Video

Updated: Apr 25, 2026

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

Skeletal defects in Osterix-Cre transgenic mice.

Wei Huang¹, Bjorn R Olsen

¹Department of Developmental Biology, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA, 02115, USA, wei_huang@hms.harvard.edu.

Transgenic Research

|August 21, 2014

Summary

Osterix-Cre (Osx-Cre) transgenic mice exhibit skeletal defects, including delayed bone formation and fracture healing. Researchers recommend using Osx-Cre controls for accurate skeletal studies involving this widely used gene-targeting tool.

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Last Updated: Apr 25, 2026

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Area of Science:

Molecular Biology
Genetics
Developmental Biology

Background:

Cre/loxP recombination is a key technology for conditional gene targeting in vivo.
This system enables significant discoveries in gene function for normal and disease biology.
Transgenic Cre mouse strains can exhibit unintended phenotypes, complicating research.

Purpose of the Study:

To investigate skeletal abnormalities in Osterix-Cre (Osx-Cre) transgenic mice.
To determine the necessity of Osx-Cre controls in skeletal research.
To highlight potential confounding factors in studies using Osx-Cre mice.

Main Methods:

Analysis of skeletal development in Osx-Cre transgenic mice.
Phenotypic characterization of bone ossification and fracture healing.
Comparative assessment of Osx-Cre mice and appropriate controls.

Main Results:

Osx-Cre mice displayed delayed calvarial ossification.
Multiple skeletal sites in Osx-Cre mice showed impaired fracture callus formation.
These findings indicate inherent skeletal defects in the Osx-Cre mouse model.

Conclusions:

Osterix-Cre (Osx-Cre) transgenic mice possess inherent skeletal defects.
Osx-Cre mice are unsuitable as controls for skeletal research without careful consideration.
Researchers must use appropriate Osx-Cre controls for in vivo and in vitro skeletal analyses.