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Updated: Apr 25, 2026

Clinical Examination Protocol to Detect Atypical and Classical Scrapie in Sheep
Published on: January 19, 2014
Allelic variants at codon 146 in the PRNP gene show significant differences in the risk for natural scrapie in
A Ortiz-Pelaez1, S Georgiadou2, M M Simmons1
1Animal Health and Veterinary Laboratories Agency,New Haw,Addlestone,Surrey,UK.
Abstract:
Previous studies have shown the association between the polymorphisms serine (S) or aspartic acid (D) at codon 146 of the PRNP gene and resistance to scrapie. All goats aged >12 months (a total of 1075 animals) from four herds with the highest prevalence of scrapie in the country were culled and tested, of which 234 (21·7%) were positive by either the rapid test or immunohistochemistry (IHC) for any of the tissues tested. The odds of scrapie infection occurring in NN146 goats was 101 [95% credible interval (CrI) 19-2938] times higher than for non-NN146 or unknown genotypes. IHC applied to lymphoreticular tissue produced the highest sensitivity (94%, 95% CrI 90-97). The presence of putatively resistant non-NN146 alleles in the Cypriot goat population, severely affected by scrapie, provides a potential tool to reduce/eradicate scrapie provided that coordinated nationwide breeding programmes are implemented and maintained over time.
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