Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predictive mechanistic modelling of vegetable oil autoxidation using a parametrised kinetic network.

Current research in food science·2025
Same author

Mechanistic kinetic model to elucidate lipid oxidation mechanisms in spray-dried microencapsulated oils.

Food chemistry·2025
Same author

Dendrimers Improve Apolipoprotein Nanoparticle mRNA Delivery to Immune Cells.

Advanced materials (Deerfield Beach, Fla.)·2025
Same author

Targeting mTOR in myeloid cells prevents infection-associated inflammation.

iScience·2025
Same author

Comprehensive quantitative profiling of vegetable oil oxidation products by NMR-based oxylipidomics.

Food research international (Ottawa, Ont.)·2025
Same author

Nature-inspired platform nanotechnology for RNA delivery to myeloid cells and their bone marrow progenitors.

Nature nanotechnology·2025

Related Experiment Video

Updated: Apr 25, 2026

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications
09:39

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

Published on: February 7, 2021

9.2K

NMR nanoparticle diffusometry in hydrogels: enhancing sensitivity and selectivity.

Daan W de Kort1, John P M van Duynhoven, Freek J M Hoeben

  • 1Laboratory of Biophysics and NMR Centre, Wageningen University , Dreijenlaan 3, 6703 HA Wageningen, The Netherlands.

Analytical Chemistry
|August 21, 2014
PubMed
Summary

Researchers developed fluorine-19 (19F) labeled nanoparticles for background-free nuclear magnetic resonance (NMR) experiments. This technique enhances the study of nanoparticle diffusion in complex hydrogels for submicron structural analysis.

More Related Videos

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study
10:10

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study

Published on: August 15, 2016

9.2K
Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
06:26

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

1.9K

Related Experiment Videos

Last Updated: Apr 25, 2026

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications
09:39

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

Published on: February 7, 2021

9.2K
Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study
10:10

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study

Published on: August 15, 2016

9.2K
Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
06:26

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

1.9K

Area of Science:

  • Materials Science
  • Polymer Chemistry
  • Nanotechnology

Background:

  • Nanoparticle diffusion in hydrogels reveals submicron polymer matrix structures.
  • Pulsed-gradient spin-echo NMR is used, but (1)H DOSY struggles with low nanoparticle signals in protonated backgrounds.
  • Developing selective and sensitive methods for nanoparticle diffusometry is crucial.

Purpose of the Study:

  • To enable background-free (19)F DOSY experiments for nanoparticle diffusometry.
  • To benchmark (19)F DOSY against (1)H DRCOSY for signal separation and sensitivity.
  • To achieve quantitative diffusometry even at low magnetic field strengths.

Main Methods:

  • Preparation of (19)F labeled, PEGylated, water-soluble dendritic nanoparticles (~7 wt % (19)F loading).
  • Implementation of (19)F DOSY experiments for nanoparticle diffusometry.
  • Benchmarking against (1)H diffusion-T2 correlation spectroscopy (DRCOSY).
  • Application of bootstrap data resampling for 2D-Laplace inversion stabilization.

Main Results:

  • Successful background-free (19)F DOSY experiments were achieved.
  • (19)F DOSY demonstrated high sensitivity and selectivity for nanoparticle diffusion.
  • Quantitative diffusometry was possible even at low magnetic field strengths (30 MHz) using stabilized DRCOSY data.

Conclusions:

  • (19)F labeled nanoparticles offer a superior approach for background-free NMR diffusometry in hydrogels.
  • The developed methods provide enhanced sensitivity and selectivity for analyzing submicron hydrogel structures.
  • This technique advances the quantitative characterization of complex polymer matrices using nanoparticle probes.