Bacterial and archaeal communities in Lake Nyos (Cameroon, Central Africa)
Rosine E Tiodjio1, Akihiro Sakatoku1, Akihiro Nakamura1
1Department of Environmental and Energy Sciences, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan.
Abstract:
The aim of this study was to assess the microbial diversity associated with Lake Nyos, a lake with an unusual chemistry in Cameroon. Water samples were collected during the dry season on March 2013. Bacterial and archaeal communities were profiled using Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) approach of the 16S rRNA gene. The results indicate a stratification of both communities along the water column. Altogether, the physico-chemical data and microbial sequences suggest a close correspondence of the potential microbial functions to the physico-chemical pattern of the lake. We also obtained evidence of a rich microbial diversity likely to include several novel microorganisms of environmental importance in the large unexplored microbial reservoir of Lake Nyos.
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