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RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Apr 25, 2026

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

Poretools: a toolkit for analyzing nanopore sequence data.

Nicholas J Loman¹, Aaron R Quinlan¹

¹Institute of Microbiology and Infection, University of Birmingham, Birmingham B15 2TT, UK and Department of Public Health Sciences, University of Virginia, Charlottesville 22932, VA, USA.

Bioinformatics (Oxford, England)

|August 22, 2014

Summary

This summary is machine-generated.

Poretools is a new open-source software toolkit designed for analyzing nanopore sequencing data from the MinION device. It offers essential tools for quality control and downstream analysis of genomic datasets.

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Last Updated: Apr 25, 2026

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

Area of Science:

Genomics
Bioinformatics

Background:

Nanopore sequencing offers a disruptive approach to genomics, enabling single DNA molecule detection without amplification.
The Oxford Nanopore Technologies (ONT) MinION™ is a portable, real-time nanopore sequencer.
A standardized toolkit for analyzing nanopore sequencing data is currently lacking in the research community.

Purpose of the Study:

To introduce poretools, a flexible software toolkit for exploring and analyzing nanopore sequencing datasets.
To facilitate quality control and downstream analysis of data generated by MinION™ devices.

Main Methods:

Poretools is an open-source software developed in Python.
It functions as both a suite of command-line utilities and a Python application programming interface.
The software operates directly on the native FAST5 file format produced by ONT devices.

Main Results:

Poretools provides essential utilities for format conversion.
It includes data exploration and visualization tools for nanopore sequencing datasets.
The toolkit supports quality control and downstream analysis of MinION™ data.

Conclusions:

Poretools offers a standardized solution for analyzing nanopore sequencing data.
This toolkit enhances the utility of MinION™ sequencing for genomic research.