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Blood coagulation screening using a paper-based microfluidic lateral flow device.

H Li¹, D Han, G M Pauletti

¹Department of Electrical Engineering and Computing Systems, College of Engineering and Applied Science, University of Cincinnati, Cincinnati, OH, USA 45221. a.steckl@uc.edu.

|August 22, 2014

Summary

This summary is machine-generated.

This study introduces a simple microfluidic paper-based lateral flow assay (LFA) for evaluating blood coagulation using whole blood. The device offers a straightforward point-of-care method for monitoring blood clotting times, utilizing red blood cell movement as a visual indicator.

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Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

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Published on: April 16, 2016

Area of Science:

Biomedical Engineering
Analytical Chemistry
Hematology

Background:

Accurate blood coagulation assessment is crucial for clinical diagnostics and patient monitoring.
Conventional coagulation analyzers can be complex and require specialized laboratory settings.
Point-of-care (POC) testing demands simple, rapid, and reliable diagnostic tools.

Purpose of the Study:

To develop and evaluate a microfluidic paper-based lateral flow assay (LFA) for simple blood coagulation evaluation.
To utilize whole blood directly, eliminating the need for plasma separation.
To establish a visual, point-of-care method for assessing blood clotting ability.

Main Methods:

A paper-based lateral flow assay (LFA) device was designed with a sample pad, analytical membrane, and wicking pad.
Whole animal blood (rabbit) was treated with trisodium citrate and varying CaCl2 concentrations to control coagulation.
The travel distance of red blood cells (RBCs) on the paper membrane was measured as an indicator of coagulation time.

Main Results:

The LFA device successfully evaluated blood coagulation using whole blood without plasma separation.
The travel distance of the RBC front showed a linear decrease with increasing CaCl2 concentration.
The RBC travel rate decreased from 3.25 mm/min (no CaCl2) to 2.2 mm/min (500 mM CaCl2).
The LFA demonstrated a larger linear range and greater simplicity compared to conventional analyzers.

Conclusions:

The developed paper-based LFA offers a simple, visual, and effective method for evaluating blood coagulation at the point-of-care.
This approach allows for rapid screening and self-monitoring of blood clotting properties using whole blood.
The RBC travel distance serves as a reliable indicator for assessing blood's coagulation status.