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In vitro granulocyte aggregation.

S Antonsen1, J M Beyer

  • 1Department of Clinical Chemistry and Medical Rheumatology, Odense University Hospital, Denmark.

Scandinavian Journal of Clinical and Laboratory Investigation
|November 1, 1989
PubMed
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Rheumatoid arthritis patients may experience transient granulocyte aggregation in EDTA-blood, causing a temporary decrease in leukocyte counts. This phenomenon, observed multiple times, involves granulocyte clumping without platelet involvement.

Area of Science:

  • Hematology
  • Immunology
  • Rheumatology

Background:

  • Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation.
  • Hematological parameters can be affected in patients with autoimmune conditions.
  • The behavior of leukocytes, particularly granulocytes, in vitro is crucial for understanding immune responses.

Observation:

  • Transient granulocyte aggregation was observed in ethylenediaminetetraacetic acid (EDTA)-treated blood samples from a patient with rheumatoid arthritis.
  • This phenomenon recurred three times over a 10-month observation period.
  • No involvement of thrombocytes (platelets) was detected in the observed aggregation.

Findings:

  • A significant decrease of approximately 30% in leukocyte count was recorded by Coulter Counter within 6 hours of blood collection.

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  • The reduction in leukocyte count was temporary and did not lead to overt leukopenia.
  • Microscopic examination of blood smears revealed a concurrent increase in the number of granulocyte aggregates.

    • Implications:

    • This observation suggests a potential in vitro artifact or a specific immune-mediated phenomenon in RA patients affecting granulocytes.
    • Understanding granulocyte aggregation in EDTA-blood may offer insights into RA pathogenesis or diagnostic markers.
    • Further research is warranted to elucidate the mechanisms and clinical significance of this transient granulocyte aggregation in rheumatoid arthritis.