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Published on: June 26, 2020
Spherical nucleic acids as a divergent platform for synthesizing RNA-nanoparticle conjugates through enzymatic
Jessica L Rouge1, Liangliang Hao, Xiaochen A Wu
1Department of Chemistry and ‡International Institute for Nanotechnology, §Interdisciplinary Biological Sciences Graduate Program, Northwestern University , 2145 Sheridan Road, Evanston, Illinois 60208-3113, United States.
Abstract:
Herein, we describe a rapid, divergent method for using spherical nucleic acids (SNAs) as a universal platform for attaching RNA to DNA-modified nanoparticles using enzyme-mediated techniques. This approach provides a sequence-specific method for the covalent attachment of one or more in vitro transcribed RNAs to a universal SNA scaffold, regardless of RNA sequence. The RNA-nanoparticle constructs are shown to effectively knock down two different gene targets using a single, dual-ligated nanoparticle construct.
