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AIMP1 negatively regulates adipogenesis by inhibiting PPARγ

Jong Hyun Kim1, Jung Ho Lee2, Min Chul Park1

  • 1Medicinal Bioconvergence Research Center, Seoul National University, Seoul 151-742, South Korea.

Journal of Cell Science
|August 23, 2014
PubMed

Insights

Aminoacyl tRNA synthetase-interacting multifunctional protein 1 (AIMP1) inhibits adipogenesis by interacting with peroxisome proliferator-activated receptor γ (PPARγ). AIMP1 prevents PPARγ transcriptional activation, regulating fat cell differentiation.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Adipogenesis is regulated by transcription factors and co-regulators, but regulatory mechanisms remain incompletely understood.
  • The roles of translational factors in adipogenesis are largely unclear.
  • Peroxisome proliferator-activated receptor γ (PPARγ) is a key transcription factor in adipogenesis.

Purpose of the Study:

  • To investigate the role of aminoacyl tRNA synthetase-interacting multifunctional protein 1 (AIMP1) in adipogenesis.
  • To elucidate the mechanism by which AIMP1 regulates adipocyte differentiation.
  • To determine the interaction between AIMP1 and PPARγ.

Main Methods:

  • Analysis of AIMP1 expression during adipocyte differentiation.
  • Assessment of adipogenesis in AIMP1-deficient cells.
  • Investigation of AIMP1 interaction with PPARγ using biochemical assays.
  • Evaluation of AIMP1's effect on PPARγ transcriptional activity.

Main Results:

  • AIMP1 expression increases during adipocyte differentiation.
  • Adipogenesis is enhanced in AIMP1-deficient cells, indicating a negative regulatory role.
  • AIMP1 directly interacts with the DNA-binding domain of active PPARγ.
  • AIMP1 inhibits the transcriptional activity of PPARγ.

Conclusions:

  • AIMP1 acts as a novel negative regulator of adipogenesis.
  • AIMP1 inhibits adipocyte differentiation by directly interacting with and suppressing PPARγ transcriptional activity.
  • AIMP1's interaction with PPARγ provides a new mechanism for controlling adipogenesis.
Keywords:
AIMP1AdipogenesisPPARγtRNA synthetase

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