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Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
Published on: March 17, 2023
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AIMP1 negatively regulates adipogenesis by inhibiting PPARγ
Jong Hyun Kim1, Jung Ho Lee2, Min Chul Park1
1Medicinal Bioconvergence Research Center, Seoul National University, Seoul 151-742, South Korea.
Journal of Cell Science
|August 23, 2014
Summary
Aminoacyl tRNA synthetase-interacting multifunctional protein 1 (AIMP1) inhibits adipogenesis by interacting with peroxisome proliferator-activated receptor γ (PPARγ). AIMP1 prevents PPARγ transcriptional activation, regulating fat cell differentiation.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Adipogenesis is regulated by transcription factors and co-regulators, but regulatory mechanisms remain incompletely understood.
- The roles of translational factors in adipogenesis are largely unclear.
- Peroxisome proliferator-activated receptor γ (PPARγ) is a key transcription factor in adipogenesis.
Purpose of the Study:
- To investigate the role of aminoacyl tRNA synthetase-interacting multifunctional protein 1 (AIMP1) in adipogenesis.
- To elucidate the mechanism by which AIMP1 regulates adipocyte differentiation.
- To determine the interaction between AIMP1 and PPARγ.
Main Methods:
- Analysis of AIMP1 expression during adipocyte differentiation.
- Assessment of adipogenesis in AIMP1-deficient cells.
- Investigation of AIMP1 interaction with PPARγ using biochemical assays.
- Evaluation of AIMP1's effect on PPARγ transcriptional activity.
Main Results:
- AIMP1 expression increases during adipocyte differentiation.
- Adipogenesis is enhanced in AIMP1-deficient cells, indicating a negative regulatory role.
- AIMP1 directly interacts with the DNA-binding domain of active PPARγ.
- AIMP1 inhibits the transcriptional activity of PPARγ.
Conclusions:
- AIMP1 acts as a novel negative regulator of adipogenesis.
- AIMP1 inhibits adipocyte differentiation by directly interacting with and suppressing PPARγ transcriptional activity.
- AIMP1's interaction with PPARγ provides a new mechanism for controlling adipogenesis.
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