Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bridging the Cultivation Gap in Plant Microbiomes: A Comparative Study of Aerial Root Mucilage Microbiome Characterization by Conventional Isolation, Prospector High-Throughput Cultivation, and Molecular Profiling.

MicrobiologyOpen·2026
Same author

Synthesis, function, and genetic variation of sorgoleone, the major biological nitrification inhibitor in sorghum.

Crop science·2025
Same author

Designing a nitrogen-efficient cold-tolerant maize for modern agricultural systems.

The Plant cell·2025
Same author

In-vivo Raman microspectroscopy reveals differential nitrate concentration in different developmental zones in Arabidopsis roots.

Plant methods·2024
Same author

Passive permeability controls synthesis for the allelochemical sorgoleone in sorghum root exudate.

Phytochemistry·2023
Same author

Developmental regulation and physical interaction among enzymes involved in sorgoleone biosynthesis.

The Plant journal : for cell and molecular biology·2023

Related Experiment Video

Updated: Apr 25, 2026

Glutamine Flux Imaging Using Genetically Encoded Sensors
10:23

Glutamine Flux Imaging Using Genetically Encoded Sensors

Published on: July 31, 2014

9.0K

Glutamine flux imaging using genetically encoded sensors.

Julien Besnard1, Sakiko Okumoto2

  • 1Virginia Tech.

Journal of Visualized Experiments : Jove
|August 23, 2014
PubMed
Summary

Genetically encoded sensors offer real-time subcellular monitoring of biological molecules. This study demonstrates observing transporter dynamics using a genetically encoded glutamine sensor for cellular transport analysis.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Genetically encoded sensors enable real-time, subcellular monitoring of biological molecules.
  • These sensors have become essential tools in molecular and cellular biology research.
  • Applications include investigating cellular transport processes with high specificity.

Purpose of the Study:

  • To demonstrate the observation of transporter dynamics using genetically encoded sensors.
  • To highlight the utility of genetically encoded glutamine sensors in studying cellular transport.
  • To provide guidance on experimental design and analysis for transporter studies.

Main Methods:

  • Utilizing genetically encoded sensors for molecular monitoring.
  • Focusing on genetically encoded glutamine sensors as a model system.

More Related Videos

FIBS-enabled Noninvasive Metabolic Profiling
09:16

FIBS-enabled Noninvasive Metabolic Profiling

Published on: February 3, 2014

9.1K
Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
07:47

Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator

Published on: October 20, 2021

2.5K

Related Experiment Videos

Last Updated: Apr 25, 2026

Glutamine Flux Imaging Using Genetically Encoded Sensors
10:23

Glutamine Flux Imaging Using Genetically Encoded Sensors

Published on: July 31, 2014

9.0K
FIBS-enabled Noninvasive Metabolic Profiling
09:16

FIBS-enabled Noninvasive Metabolic Profiling

Published on: February 3, 2014

9.1K
Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
07:47

Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator

Published on: October 20, 2021

2.5K
  • Detailed discussion of experimental design and technical settings.

    • Main Results:

    • Successful observation of transporter dynamics.
    • Demonstration of cell-type specific transport activity analysis.
    • Insights into transporter kinetics and substrate specificities.

    Conclusions:

    • Genetically encoded sensors are powerful tools for studying cellular transport.
    • The glutamine sensor exemplifies the potential for real-time analysis of transporter function.
    • Methodologies discussed facilitate advanced investigations into cellular dynamics.