Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Restarting Stalled Replication Forks

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Using hierarchical Bayesian modelling to assess shedder test suitability.

Forensic science international. Genetics·2026

Same author

High-level data fusion using majority voting for the classification of spray paint spectroscopic data.

Forensic science international·2026

Same author

Probabilistic searching of databases compared to binary CODIS eligibility: An improved methodology for maximizing investigative leads using short tandem repeat DNA profiles.

Forensic science international. Genetics·2026

Same author

An adaptive weighted polynomial baseline correction method for electrochemical aptamer-based sensors.

The Analyst·2026

Same author

deepNoC: A deep learning system to assign the number of contributors to a short tandem repeat DNA profile.

Forensic science international. Genetics·2026

Same author

An evaluation of ForenSeq DNA Signature Prep iiSNP mixture interpretation using a probabilistic genotyping method.

Forensic science international. Genetics·2026

Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026

Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026

Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026

Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026

Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026

Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 25, 2026

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat VNTR - Fragment Length Analysis FLA

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat VNTR - Fragment Length Analysis FLA

Published on: July 15, 2011

Modeling forward stutter: toward increased objectivity in forensic DNA interpretation.

Jo-Anne Bright¹, John S Buckleton, Duncan Taylor

¹ESR Ltd., Auckland, New Zealand; Department of Statistics, University of Auckland, Auckland, New Zealand.

Electrophoresis

|August 23, 2014

Summary

This summary is machine-generated.

Forward stutter, a PCR artifact in forensic DNA analysis, is generally unpredictable for most STRs. However, for the D22S1045 locus, high forward stutter correlates with high backward stutter.

Keywords:

Continuous interpretation models Forensic DNA Forward stutter Interpretation

More Related Videos

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

Related Experiment Videos

Last Updated: Apr 25, 2026

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat VNTR - Fragment Length Analysis FLA

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat VNTR - Fragment Length Analysis FLA

Published on: July 15, 2011

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

Area of Science:

Forensic Genetics
Molecular Biology
Polymerase Chain Reaction (PCR) analysis

Background:

Forward stutter, an artifact in short tandem repeat (STR) analysis, is a rare PCR product.
Understanding forward stutter is crucial for accurate forensic DNA profiling.

Purpose of the Study:

Investigate factors influencing forward stutter occurrence and size in autosomal multiplex STR analysis.
Evaluate models for predicting forward stutter peak heights.

Main Methods:

Analysis of four autosomal multiplexes.
Statistical modeling to identify explanatory variables for forward stutter.
Examination of correlations between stutter, allelic peak height, marker, and allele characteristics.

Main Results:

No significant correlation found for tetra- and penta-nucleotide repeats regarding peak height, marker, or allele sequence.
Forward stutter for these repeat types fits a gamma distribution without explanatory variables.
For the trinucleotide repeat D22S1045, forward stutter is more frequent.
Backward stutter height emerged as the best predictor for forward stutter at D22S1045.

Conclusions:

Forward stutter in most STRs is not predictable by common variables.
A potential co-causation mechanism links high forward and backward stutter at the D22S1045 locus.