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Updated: Apr 25, 2026

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Published on: March 22, 2020

Fluorescent carbon dot (C-dot) nanoclusters.

Guan Wang¹, Xiaoyong Pan, Liuqun Gu

¹Institute of Materials Research and Engineering, A *STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore 117602.

|August 23, 2014

Summary

This summary is machine-generated.

Researchers developed novel fluorescent carbon dot nanoclusters for enhanced bioimaging. These carbon dot nanoclusters offer brighter, more even cell imaging with improved photostability and low cytotoxicity.

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Area of Science:

Nanomaterials Science
Biotechnology
Chemical Engineering

Background:

Carbon dots (C-dots) are emerging nanomaterials with unique optical properties.
Hollow silica spheres offer a versatile platform for nanomaterial loading and functionalization.
Developing advanced C-dot formulations is crucial for improving bioimaging resolution and sensitivity.

Purpose of the Study:

To synthesize and characterize novel fluorescent carbon dot nanoclusters.
To evaluate the bioimaging capabilities of these nanoclusters in live cells.
To assess the potential of C-dot nanoclusters for enhanced cellular visualization.

Main Methods:

Synthesis of C-dot nanoclusters via dehydration of mannose adsorbed onto hollow silica spheres or PEG-g-hollow silica.
Structural characterization using techniques such as 1H NMR, FTIR, TEM, and TGA.
Evaluation of fluorescence properties, quantum yield, and cellular imaging using confocal laser scanning microscopy (CLSM).

Main Results:

Successful formation of C-dot nanoclusters with redshifted fluorescence emission.
Quantum yields improved to approximately 2% after passivation with PEG diamines.
C-dot nanoclusters provided brighter and more uniform cytoplasm imaging in live Hep G2 and MCF-7 cells compared to free C-dots.

Conclusions:

C-dot nanoclusters exhibit promising photostability and low cytotoxicity.
These nanoclusters demonstrate superior performance for cytoplasm bioimaging.
The developed C-dot nanoclusters represent a significant advancement for high-quality cellular imaging applications.