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Humanized Mediator Release Assay as a Read-Out for Allergen Potency
Published on: June 29, 2021
Measuring histamine and cytokine release from basophils and mast cells
Bettina M Jensen1, Sidsel Falkencrone, Per S Skov
1Allergy Clinic, Dept. 22, 1. floor, Gentofte Hospital, Niels Andersens vej 65, 2900, Hellerup, Denmark, bettina.margrethe.jensen@regionh.dk.
Abstract:
Basophils and mast cells are known for their capability to release both preformed and newly synthesized inflammatory mediators. In this chapter we describe how to stimulate and detect histamine released from basophils in whole blood, purified basophils, in vitro cultured mast cells, and in situ skin mast cells. We also give an example of an activation protocol for basophil and mast cell cytokine release and discuss approaches for cytokine detection.
