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Basophil Activation Test for Allergy Diagnosis
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Flow cytometric allergy diagnosis: basophil activation techniques.

Chris H Bridts1, Vito Sabato, Christel Mertens

  • 1Department of Immunology and Allergology, University of Antwerp, Campus Drie Eiken, D.T.596, Universiteitsplein 1, 2610, Wilrijk, Belgium.

Methods in Molecular Biology (Clifton, N.J.)
|August 24, 2014
PubMed
Summary

Flow cytometry offers a new method for allergy diagnosis by measuring basophil activation. This technique quantifies changes in surface markers and histamine release, aiding in allergy detection.

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Area of Science:

  • Immunology
  • Allergy Diagnostics
  • Flow Cytometry

Background:

  • Basophil activation is key to allergic reactions.
  • Flow cytometry detects changes in basophil surface markers like CD63 and CD203c.
  • These changes are linked to allergen exposure and IgE-mediated responses.

Purpose of the Study:

  • To present two flow cytometry protocols for assessing basophil activation.
  • To measure surface marker upregulation and intragranular histamine content.
  • To provide methods for enhanced allergy diagnosis.

Main Methods:

  • Utilizing multicolor flow cytometry with specific monoclonal antibodies.
  • Protocol 1: Detecting surface marker activation on basophils.
  • Protocol 2: Quantifying intragranular histamine release from basophils.

Main Results:

  • The described methods allow for the detection of distinct facets of basophil activation.
  • Surface marker expression changes reflect allergen-specific immune responses.
  • Histamine content alterations provide another measure of basophil degranulation.

Conclusions:

  • Flow cytometry provides a robust platform for basophil activation analysis.
  • These protocols can be applied to allergy diagnosis and research.
  • Quantification of basophil activation markers and mediators enhances understanding of allergic mechanisms.