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Isocitrate dehydrogenase from thermophilic and mesophilic bacteria. Isolation and some characteristics
1Department of Biochemistry, University of Manchester Institute of Science & Technology, England.
The International Journal of Biochemistry
|January 1, 1989
Abstract:
1. Simple methods incorporating the principle of selective enzyme elution from a triazinyl dye adsorbent with a mixture of NADP+ and isocitrate are described for isolating NADP+-linked isocitrate dehydrogenase in pure state from several mesophilic and thermophilic bacteria. 2. Several characteristics of the isocitrate dehydrogenases have been examined, viz. molecular size, amino acid composition including the content of sulphydryl groups, thermostability and structural homology by the criterion of immunological cross-section.