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Related Experiment Video

Updated: Apr 25, 2026

Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis
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T cell isolation from mouse kidneys.

Isis Ludwig-Portugall1, Christian Kurts

  • 1Institute of Experimental Immunology, Rheinische Friedrich-Wilhelms-University of Bonn, Sigmund-Freud-Str. 25, 53105, Bonn, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2014
PubMed
Summary

Researchers developed a new method to isolate T cells from mouse kidneys. This technique aids in understanding immune-mediated kidney diseases and T cell roles in nephritis.

Area of Science:

  • Immunology
  • Nephrology
  • Cell Biology

Background:

  • Kidneys normally have few lymphocytes, with ~1-5 × 10^3 CD4(+) T cells per healthy mouse kidney.
  • Immune-mediated kidney diseases involve infiltration of various T cell subsets, including γδ T cells, NKT cells, CD4(+) T cells, CD8(+) T cells, and regulatory T cells (Tregs).
  • The composition and number of infiltrating T cells differ across nephritis types, with CD4(+) T cells implicated in glomerulonephritis-induced renal injury via cytokine production and B cell help.

Purpose of the Study:

  • To develop an effective and specific protocol for isolating T cells from murine kidneys.
  • To facilitate ex vivo analysis of T cells infiltrating the kidney.
  • To advance the understanding of pathomechanisms in immune-mediated kidney diseases.

Main Methods:

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  • Development of a novel isolation protocol for T cells from the murine kidney.
  • Validation of the protocol for effectiveness and specificity.
  • Application of the protocol for ex vivo T cell analysis.

Main Results:

  • An effective and specific protocol for isolating T cells from the murine kidney was established.
  • The protocol enables detailed ex vivo analysis of kidney-infiltrating T cells.
  • This method supports research into the role of T cells in kidney diseases.

Conclusions:

  • A new T cell isolation protocol for murine kidneys has been successfully developed.
  • This protocol is crucial for studying the role of T cells in immune-mediated kidney diseases.
  • The method will aid in elucidating T cell functions in various forms of nephritis.