Intracellular staining for cytokines and transcription factors
1Institute of Experimental Immunology, University of Zürich, Winterthurerstrasse 190, 8057, Zürich, Switzerland, mair@immunology.uzh.ch.
Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2014
Abstract:
Within the past years immune cells in general and T cells in particular have been categorized into a vast variety of subsets with different functional properties. One of the key technologies fueling this emerging complexity is intracellular staining for effector cytokines and/or lineage-defining transcription factors. Here we discuss the critical steps for performing successful multicolor immunophenotyping of mouse T cells in combination with analysis of intracellular molecules after ex vivo isolation.
