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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Apr 25, 2026

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

Mass cytometry analysis of human T cell phenotype and function.

Evan W Newell¹, Lai Li Yun

¹Singapore Immunology Network (SIgN), Agency for Science Technology and Research (A-STAR), 8A Biomedical Grove, Immunos #03-06, Singapore, 138648, Singapore, evan_newell@immunol.a-star.edu.sg.

Methods in Molecular Biology (Clifton, N.J.)

|August 25, 2014

Summary

Mass cytometry, a single-cell mass spectrometry technique, enables high-dimensional analysis of cellular phenotypes. This study details its application for analyzing human T cell surface markers and intracellular cytokines.

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Area of Science:

Immunology
Biotechnology
Analytical Chemistry

Background:

Flow cytometry is a standard technique for cell analysis.
High-dimensional analysis requires methods with reduced channel cross-talk and high parameter capacity.

Purpose of the Study:

To describe methods for applying mass cytometry to human T cell analysis.
To demonstrate the utility of mass cytometry for studying T cell surface markers and intracellular cytokines.

Main Methods:

Mass cytometry utilizes single-cell mass spectrometry with elemental labels.
This technique allows for the measurement of over 30 independent cellular parameters.
Application focused on human T cell surface markers and intracellular cytokine detection.

Main Results:

Mass cytometry offers reduced cross-talk compared to traditional flow cytometry.
The method enables comprehensive, high-dimensional profiling of T cell populations.
Successful analysis of T cell surface markers and intracellular cytokine expression was achieved.

Conclusions:

Mass cytometry is a powerful approach for high-dimensional analysis of immune cells.
The described methods facilitate detailed characterization of human T cell phenotypes and functions.
This technique enhances the understanding of cellular processes in immunology research.