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Updated: Apr 25, 2026

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Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice
Published on: July 11, 2016
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A method for the generation of TCR retrogenic mice
Elisa Kieback1, Ellen Hilgenberg, Simon Fillatreau
1Max Delbrueck Center for Molecular Medicine, Berlin, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2014
Abstract:
Retrogenic mice provide a unique system for rapidly analyzing the function of genes in the hematopoietic system. Here, we provide a detailed protocol for the production of retrogenic mice expressing genes coding for T cell receptor (TCR) for antigen. This technology should be easy to establish in any laboratory and should allow for a rapid progress in our understanding of the functional roles of TCR repertoires in immunity.

