Related Experiment Video

Updated: Apr 25, 2026

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
10:00

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

27.9K

Specific siRNA inhibits XIAP expression in human endometrial carcinoma cell apoptosis

Duo Yin1, Ning Wang, Shu-lan Zhang

  • 1Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, 36 Sanhao Road, Heping District, Shenyang, 110004, Liaoning Province, China.

Cell Biochemistry and Biophysics
|August 25, 2014
PubMed

Insights

Small interfering RNA (siRNA) effectively inhibited X-linked inhibitor of apoptosis protein (XIAP) gene expression in human endometrial cancer cells. This inhibition significantly promoted cancer cell apoptosis, suggesting a potential therapeutic strategy.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Therapy

Background:

  • Endometrial carcinoma is a significant gynecological malignancy.
  • X-linked inhibitor of apoptosis protein (XIAP) is implicated in cancer cell survival and resistance to apoptosis.
  • Targeting XIAP offers a potential strategy for endometrial cancer treatment.

Purpose of the Study:

  • To investigate the inhibitory effects of RNA interference targeting XIAP on human endometrial carcinoma RL95-2 cells.
  • To assess the impact of XIAP gene silencing on cell apoptosis and proliferation.

Main Methods:

  • Design and synthesis of small interfering RNA (siRNA) specific for XIAP.
  • Transfection of RL95-2 cells with XIAP siRNA.
  • Quantitative assessment of XIAP mRNA levels using real-time PCR.
  • Assessment of XIAP protein expression via Western Blotting.
  • Evaluation of cell proliferation and apoptosis using MTT assay and flow cytometry.

Main Results:

  • XIAP siRNA significantly reduced XIAP mRNA and protein expression in RL95-2 cells (P < 0.05).
  • A significant increase in cell growth inhibition rate (47.86 ± 4.46%) was observed in the siRNA-treated group (P < 0.05).
  • Flow cytometry analysis indicated a significant promotion of apoptosis in endometrial carcinoma cells following XIAP gene silencing.

Conclusions:

  • Synthetic siRNA effectively inhibits XIAP gene expression at both mRNA and protein levels in human endometrial carcinoma RL95-2 cells.
  • XIAP gene silencing significantly promotes apoptosis of endometrial carcinoma cells.
  • Further investigation is warranted to elucidate the precise mechanisms underlying XIAP-mediated apoptosis in this context.

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

6.8K
Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms13:40

Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms

Published on: July 7, 2011

20.0K

Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
13.4K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.5K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
20.9K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.2K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.0K