Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

6.2K
6.2K
Homologous Recombination02:31

Homologous Recombination

58.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
58.7K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.7K
Crossing Over01:30

Crossing Over

6.3K
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
6.3K
Gene Conversion02:08

Gene Conversion

9.2K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
9.2K
Exon Recombination02:32

Exon Recombination

3.1K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantifying donor-recipient mismatches using recipient-derived sources of donor DNA.

medRxiv : the preprint server for health sciences·2026
Same author

APOL1-risk alleles modulate T-cell receptor signaling to promote allograft rejection.

The Journal of clinical investigation·2026
Same author

Brain-wide Genome Editing via STEP-RNPs for Treatment of Angelman Syndrome.

bioRxiv : the preprint server for biology·2025
Same author

A T-cell intrinsic Role for <i>APOL1</i> Risk Alleles in Allograft Rejection.

bioRxiv : the preprint server for biology·2025
Same author

Biology of Hox Genes: Questions and Technological Challenges.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Optimized smFISH Pipeline for Studying Nascent Transcription in Mouse Embryonic Tissue Samples.

Methods in molecular biology (Clifton, N.J.)·2025

Related Experiment Video

Updated: Apr 25, 2026

Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

18.5K

Hox complex analysis through BAC recombineering.

Mark Parrish1, Youngwook Ahn, Christof Nolte

  • 1Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, MO, 64110, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2014
PubMed
Summary
This summary is machine-generated.

Bacterial artificial chromosome (BAC) transgenesis in mice enables detailed study of Hox gene regulation and function. Recombineering tools allow precise manipulation of these large DNA constructs for advanced Hox biology research.

More Related Videos

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

7.7K
Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

17.3K

Related Experiment Videos

Last Updated: Apr 25, 2026

Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

18.5K
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

7.7K
Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

17.3K

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Hox complexes are crucial for animal development.
  • Studying Hox gene regulation requires large DNA constructs.
  • Bacterial artificial chromosomes (BACs) are suitable for housing these large constructs.

Purpose of the Study:

  • To outline methods for manipulating Hox complex BACs.
  • To describe the generation of transgenic mice carrying these BACs.
  • To highlight the use of these resources in Hox biology research.

Main Methods:

  • BAC transgenesis in mice.
  • Recombineering techniques for precise DNA manipulation.
  • Generation and analysis of transgenic animal models.

Main Results:

  • BAC transgenesis allows comprehensive exploration of Hox regulatory elements.
  • Recombineering enables diverse and precise modifications of large DNA constructs.
  • Transgenic mice provide valuable tools for studying Hox gene function.

Conclusions:

  • BAC transgenesis combined with recombineering offers a powerful approach to investigate Hox gene complexes.
  • These methodologies facilitate the study of fundamental aspects of Hox gene regulation and function.
  • The generated transgenic resources are essential for advancing the field of developmental genetics.