Related Experiment Video
Updated: Apr 25, 2026
Demonstration of the Rat Ischemic Skin Wound Model
Published on: April 1, 2015
Leech therapy for linear incisional skin-wound healing in rats
Kaveh Darabi Darestani1, Sayid Mahdi Mirghazanfari2, Katayoun Gohari Moghaddam3
1Department of Zoology, School of Biology, Faculty of Science, University of Tehran, Tehran, Iran.
Background:
The aim of this study was to investigate the effects of leech therapy (LT), in comparison with topical phenytoin (PHT), on incisional skin-wound healing in animal models.
Methods:
This experimental study included 15 male rats (5 animals in 3 groups) with approximately equal body weights (350 ± 10 g). Skin wounds with lengths of 20 mm and depths of 0.5 mm were made on the dorsolateral region of rats 4 cm from the spine. The first group (PHT group) was treated daily with topical PHT (1%) while the second group (LT group) received LT at the beginning of the experiment. The control group received neither the drug nor the therapy. Wound healing was evaluated every day, and the study was continued until the wound had completely healed. Changes in the areas and the appearances of the skin wounds and histological differences (at the end of the experiment) were used to investigate the differences in wound healing among the groups.
Results:
The process of wound healing was significantly faster in the group treated with LT (p < 0.05) than in the group treated with the PHT.
Conclusion:
The study results showed that LT improved incisional skin-wound healing in rats.
