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Updated: Apr 25, 2026
Pull-down of Calmodulin-binding Proteins
Published on: January 23, 2012
Ca2+-controlled assembly for visualized detection of conformation changes of calmodulin
Hongbo Yuan1, Chengfen Xing, Hailong An
1Key Laboratory of Hebei Province for Molecular Biophysics, Institute of Biophysics, Hebei University of Technology , Tianjin 300401, P. R. China.
Abstract:
A new strategy has been designed for visualized detection of the conformation changes of calmodulin bound to target peptide (CaM-M13) based on the conformation sensitive property of a water-soluble conjugated polythiophene derivative (PMNT) and the electrostatic interactions of PMNT/CaM-M13. Interestingly, the direct visualized PMNT color changes under UV irradiation and the turbidity changes of samples in aqueous medium can be applied to detect the conformation changes as well as the controllable assembly of PMNT/CaM-M13 with Ca(2+) in aqueous medium. Because of the specific binding of Ca(2+), the assembly of PMNT/CaM-M13 can be applied to sense calcium as well.
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