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D-xylose utilization by Saccharomyces cerevisiae.

C van Zyl1, B A Prior, S G Kilian

  • 1Department of Microbiology, University of the Orange Free State, Bloemfontein South Africa.

Journal of General Microbiology
|November 1, 1989
PubMed
Summary

Saccharomyces cerevisiae can utilize D-xylose when other substrates are present, particularly sugars like D-ribose. This yeast strain primarily dissimilates xylose, with some complete oxidation occurring under aerobic conditions.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Yeast Metabolism

Background:

  • Saccharomyces cerevisiae is widely considered unable to assimilate D-xylose.
  • Understanding yeast substrate utilization is crucial for industrial biotechnology and metabolic engineering.

Purpose of the Study:

  • To investigate the conditions under which Saccharomyces cerevisiae can utilize D-xylose.
  • To characterize the metabolic fate of D-xylose when co-metabolized with other substrates.

Main Methods:

  • Aerobic and anaerobic cultivation of Saccharomyces cerevisiae strains with D-xylose and various co-substrates.
  • Quantification of D-xylose utilization and analysis of metabolic products using radiolabeling ([14C]xylose).

Main Results:

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  • Four Saccharomyces cerevisiae strains showed varying efficiencies in D-xylose utilization, dependent on co-substrates.
  • D-xylose utilization was highest when co-metabolized with sugar substrates like D-ribose (up to 69% over 7 days).
  • Under aerobic conditions with ribose, [14C]xylose was mainly found in xylitol (91.3%), CO2 (2.6%), and biomass (1.7%), indicating dissimilation and partial oxidation.

    • Conclusions:

    • Saccharomyces cerevisiae can utilize D-xylose, contrary to general assumptions, especially when provided with suitable co-substrates.
    • The yeast primarily dissimilates xylose, but possesses a pathway for its complete oxidation in the presence of other substrates.
    • Co-metabolism strategies can enhance xylose utilization by Saccharomyces cerevisiae.