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Updated: Jan 28, 2026

Optimized and Simplified Technique for the Production and Culture of Precision-Cut Liver Slices
Published on: November 22, 2024
Precision-cut luteal slices: a promising approach for studying luteal function in pigs
Emilia Przygrodzka1, Marcelina Lopinska1, Adam J Ziecik1
1Department of Hormonal Action Mechanisms, Institute of Animal Reproduction and Food Research of PAS, Bydgoska 7 Str., 10-243 Olsztyn, Poland.
Abstract:
The study was aimed to validate the precision-cut luteal slices to investigate porcine luteal function. Corpora lutea (CLs) were cut into 180-μm thick slices using Krumdick Tissue Slicer. The viability, tissue structure and steroidogenic acute regulatory protein (STAR) expression in the luteal slices did not differ between the beginning and the end of the 24-h incubation period. The luteal progesterone secretion showed a time- and dose-dependent response to porcine luteinizing hormone. The effects of prostaglandin F(2α) and 17β-estradiol on progesterone secretion by porcine luteal slices were comparable to the previously reported in vivo results of the CL microdialysis system in the pig.
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