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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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Raman Spectroscopy Instrumentation: Overview01:26

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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Applications Of NMR In Biology01:25

Applications Of NMR In Biology

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Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
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Probing the Interaction at the Nano-Bio Interface Using Raman Spectroscopy: ZnO Nanoparticles and Adenosine

A Bhaumik1, A M Shearin1, R Delong1

  • 1Department of Physics, Astronomy and Materials Science, Department of Biomedical Science, and Department of Chemistry, Missouri State University , Springfield, Missouri 65897, United States.

The Journal of Physical Chemistry. C, Nanomaterials and Interfaces
|August 26, 2014
PubMed
Summary
This summary is machine-generated.

Researchers explored interactions between zinc oxide (ZnO) nanostructures and adenosine triphosphate (ATP). Raman spectroscopy revealed strong ZnO binding to ATP

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Area of Science:

  • Nanobiotechnology
  • Biochemistry
  • Materials Science

Background:

  • Nanobiotechnology involves increasing interactions between nanomaterials and biomolecules.
  • Understanding nanoparticle-biomaterial interactions is crucial for molecular-level life processes.
  • Complex biophysicochemical reactions govern nano-bio boundaries.

Purpose of the Study:

  • To investigate the interactions between adenosine triphosphate (ATP) and zinc oxide (ZnO) nanostructures.
  • To elucidate the binding mechanisms at the nano-bio interface.
  • To demonstrate a novel method for probing these interactions.

Main Methods:

  • Hydrothermal synthesis of ZnO nanostructures.
  • Micro Raman spectroscopy for interaction analysis.
  • X-ray diffraction and electron microscopy for structural characterization.

Main Results:

  • ZnO nanostructures were shown to interact strongly with the nitrogen (N7) atom in the adenine ring of ATP.
  • Raman spectroscopy confirmed pH-dependent hydrogen bonding and phosphate group ionization.
  • Calculated molecular bond force constants reinforced experimental findings.

Conclusions:

  • Convincing evidence of pH-dependent interactions between ATP and ZnO nanomaterials was presented.
  • Raman spectroscopy is a powerful tool for studying subtle nano-bio interactions.
  • This method can be applied to elucidate the nano-bio interface more broadly.