Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

11.4K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Red-Light-Only Control of Protein-Protein Interactions Using a Cyanobacteriochrome (UNICYCL).

ACS central science·2026
Same author

Optogenetic BlueGENEs engineered into a human safe harbor locus.

Nucleic acids research·2026
Same author

Genetically Encoded SpyTag Enables Modular AAV Retargeting via SpyCatcher-Fused Ligands for Targeted Gene Delivery.

ACS synthetic biology·2025
Same author

Self-assembling information-processing biomaterial circuits.

Nature chemical biology·2025
Same author

Activation of NF-κB Signaling by Optogenetic Clustering of IKKα and β.

Advanced biology·2025
Same author

Synthetic deconvolution of an auxin-dependent transcriptional code.

Cell·2025

Related Experiment Video

Updated: Apr 25, 2026

Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression
09:08

Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression

Published on: November 4, 2025

544

Optogenetics for gene expression in mammalian cells.

Konrad Müller, Sebastian Naumann, Wilfried Weber

    Biological Chemistry
    |August 26, 2014
    PubMed
    Summary
    This summary is machine-generated.

    Optogenetic tools offer precise control over cellular processes using light, overcoming limitations of chemical inducers. This review guides selecting light-controlled gene expression systems for advanced mammalian cell biology research.

    More Related Videos

    Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
    09:20

    Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

    Published on: July 6, 2021

    2.3K
    An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
    07:59

    An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

    Published on: March 22, 2018

    6.6K

    Related Experiment Videos

    Last Updated: Apr 25, 2026

    Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression
    09:08

    Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression

    Published on: November 4, 2025

    544
    Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
    09:20

    Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

    Published on: July 6, 2021

    2.3K
    An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
    07:59

    An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

    Published on: March 22, 2018

    6.6K

    Area of Science:

    • Mammalian cell biology
    • Molecular biology
    • Biotechnology

    Background:

    • Chemical-controlled molecular switches are vital in cell biology but lack spatiotemporal precision.
    • Freely diffusing chemical inducers limit the resolution of molecular tools.
    • Optogenetics offers a solution with light-controlled, genetically encoded tools.

    Purpose of the Study:

    • To review available optogenetic tools for controlling cellular processes.
    • To highlight recent advancements in light-controlled gene expression.
    • To provide a guideline for selecting appropriate gene expression systems.

    Main Methods:

    • Overview of existing optogenetic tools.
    • Focus on recent developments in light-controlled gene expression.
    • Comparative analysis for system selection.

    Main Results:

    • Optogenetic tools provide high spatiotemporal resolution.
    • Light-controlled systems offer precise regulation of cellular functions.
    • Emerging technologies enhance control over gene expression.

    Conclusions:

    • Optogenetics revolutionizes control over cellular mechanisms.
    • Light-inducible systems are superior for precise biological research.
    • Guidance aids researchers in choosing optimal gene expression tools.