Related Experiment Video

Updated: Apr 25, 2026

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

10.3K

Kallikreins are involved in an miRNA network that contributes to prostate cancer progression

Sara Samaan, Zsuzsanna Lichner, Qiang Ding

    Biological Chemistry
    |August 26, 2014
    PubMed

    Insights

    MicroRNAs (miRNAs) regulate genes in prostate cancer. This study reveals a miRNA-kallikrein network, identifying key interactions that influence tumor progression and biochemical failure.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Oncology

    Background:

    • MicroRNAs (miRNAs) are crucial regulators of gene expression, often dysregulated in various cancers.
    • Kallikreins (KLKs) are serine proteases implicated in prostate cancer development and progression.
    • Understanding the interplay between miRNAs and KLKs is vital for deciphering prostate cancer pathogenesis.

    Purpose of the Study:

    • To elucidate the miRNA-kallikrein network involved in prostate cancer progression.
    • To identify specific miRNAs and kallikreins associated with biochemical failure and Gleason grades.
    • To investigate the functional interactions and regulatory mechanisms within this network.

    Main Methods:

    • Bioinformatic target prediction analysis to identify miRNA-kallikrein interactions.
    • In-silico and experimental validation of predicted miRNA-gene interactions.
    • Analysis of minisatellite sequences within kallikrein loci for miRNA response elements.
    • Validation of miRNA-kallikrein interactions in prostate cancer cell lines.

    Main Results:

    • Identification of 23 miRNAs dysregulated in high vs. low risk biochemical failure, targeting KLK2, KLK3, KLK4, KLK14, and KLK15.
    • Identification of 14 miRNAs differentially expressed between Gleason grades, targeting the same kallikreins.
    • Demonstration of an integrated circuit involving miR-378/422a, PIK3CG, GRB2, AKT3, KLK4, and KLK14.
    • Discovery of shared microsatellite repeats in kallikrein loci acting as predicted miRNA response elements.
    • Experimental validation of selected miRNA-kallikrein interactions in prostate cancer cell lines.

    Conclusions:

    • Kallikreins act as downstream effectors for miRNA-mediated regulation of prostate cancer progression.
    • A complex miRNA-kallikrein regulatory network significantly influences prostate cancer aggressiveness.
    • Minisatellite sequences in kallikrein genes may play a role in miRNA targeting and cross-talk.
    • Preliminary evidence suggests miRNA-mediated cross-talk exists between kallikreins.

    More Related Videos

    MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR08:30

    MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

    Published on: May 16, 2012

    24.0K
    Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients12:13

    Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

    Published on: November 19, 2019

    5.8K

    Related Concept Videos

    MicroRNAs01:22

    MicroRNAs

    MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
    3.0K
    MicroRNAs01:22

    MicroRNAs

    MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
    20.9K
    mTOR Signaling and Cancer Progression03:03

    mTOR Signaling and Cancer Progression

    The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
    The mTOR pathway or the...
    3.6K
    Abnormal Proliferation02:23

    Abnormal Proliferation

    Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
    4.0K
    The Tumor Microenvironment02:17

    The Tumor Microenvironment

    Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
    6.3K