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Updated: Aug 19, 2026

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
Published on: September 11, 2017
Transcriptional regulation of cell division genes in Escherichia coli
S J Dewar1, V Kagan-Zur, K J Begg
1Department of Molecular Biology, University of Edinburgh, UK.
Abstract:
The complete Escherichia coli ftsQ coding sequence, together with part of the ftsA coding sequence, has been cloned upstream of the lacZ open reading frame in a lambda-vector (lambda JFL100). Cells which are lysogenic for lambda JFL100 transcribe the cloned lacZ from promoter(s) within the ftsQ and ftsA sequences. The level of beta-galactosidase produced is dependent on growth rate (and/or cell size) and is derepressed in an ftsA-deficient mutant. Transcription during the cell cycle is restricted to the time of cell division.
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