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Updated: Apr 25, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
Published on: May 23, 2018
Optimization of ELFSE DNA sequencing with EOF counterflow and microfluidics
Max A Fahrenkopf1, Tamal Mukherjee, B Erik Ydstie
1Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.
Abstract:
We present a nonlinear optimization study of different implementations of the DNA electrophoretic method "End-labeled Free-solution Electrophoresis" in commercial capillary electrophoresis systems and microfluidics to improve the time required for readout. Here, the effect of electro-osmotic counterflows and snap-shot detection are considered to allow for detection of peaks soon after they are electorphoretically resolved. Using drag tags available in micelle form, we identify a design capable of sequencing 600 bases in 2.8 min.
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