Related Experiment Video
Updated: Aug 17, 2026

Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
Published on: September 20, 2011
G protein-dependent regulation of phospholipase C
Abstract:
A wide variety of receptors for hormones, neurotransmitters and growth factors control cell function via the GTP-dependent activation of phosphoinositide specific phospholipase C (PIC). At least two distinct GTP dependent proteins (G proteins) have been implicated in coupling different receptor populations to the activation of PIC and five immunologically distinct isozymes of PIC have been purified to homogeneity, prompting speculation about the potential for multiple modes of organization of the participants in this signal transduction pathway. The mechanism of hormone and G protein-dependent regulation of PIC has been studied in detail using [3H]inositol labelled turkey erythrocyte membranes and these experiments have provided strong support for the involvement of a heterotrimeric G protein. Further progress requires the development of reconstitution assays in which the regulation of isolated PICs by defined G proteins can be demonstrated.
More Related Videos
07:41A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
Published on: February 20, 2018
10:31A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
Published on: September 26, 2025
Related Concept Videos
Phosphoinositides and PIPs
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Amplifying Signals via Second Messengers
Amplifying Signals via Enzymatic Cascade
Activation and Inactivation of G Proteins
GPCRs Regulate Adenylyl Cylase Activity
Two...
IP3/DAG Signaling Pathway