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Technical Refinement of a Bilateral Renal Ischemia-Reperfusion Mouse Model for Acute Kidney Injury Research
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Aged kidneys are refractory to ischemic postconditioning in a rat model
Hui Chen1, Bianzhi Xing, Lei Wang
1Department of Urology, Renmin Hospital of Wuhan University , Wuhan , China and.
Renal Failure
|August 27, 2014
Summary
Ischemic postconditioning (IPoC) protects young kidneys from ischemia/reperfusion injury. However, this protective effect is lost in aged kidneys, possibly due to increased apoptosis.
Area of Science:
- Nephrology
- Gerontology
- Cardiovascular Research
Background:
- Ischemic postconditioning (IPoC) attenuates renal damage from ischemia/reperfusion (I/R) injury in adult models.
- The efficacy of IPoC in aging kidneys remains uninvestigated.
Purpose of the Study:
- To determine if IPoC maintains its protective effects in aging kidneys.
Main Methods:
- Aged (24-month-old) and young (3-month-old) Wistar rats underwent 45-minute renal ischemia with or without IPoC.
- Renal function (serum urea nitrogen, creatinine), histology, apoptosis, and oxidative stress were assessed.
Main Results:
- IPoC significantly reduced renal dysfunction and apoptosis in young rat kidneys post-I/R.
- IPoC failed to attenuate renal dysfunction in aged rat kidneys, correlating with increased apoptosis.
Conclusions:
- IPoC is ineffective in protecting aged rat kidneys against I/R injury.
- Severe apoptosis in aged kidneys may render them refractory to the anti-apoptotic effects of IPoC.
