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Related Experiment Video

Updated: Apr 25, 2026

Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain

Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain

Published on: March 8, 2024

Protein expression dynamics during postnatal mouse brain development.

Annelies Laeremans¹, Babs Van de Plas¹, Stefan Clerens²

¹Laboratory of Neuroplasticity and Neuroproteomics, KU Leuven, Leuven, Belgium.

Journal of Experimental Neuroscience

|August 27, 2014

Summary

This summary is machine-generated.

This study reveals key protein changes in the developing mouse brain, highlighting proteolysis and neurite outgrowth as vital for early brain maturation. These findings offer insights into nervous system development.

Keywords:

2-D DIGE biological process brain development mouse proteomics

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Last Updated: Apr 25, 2026

Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain

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Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

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Published on: February 9, 2017

Area of Science:

Neuroscience
Developmental Biology
Proteomics

Background:

Postnatal brain development involves complex molecular changes.
Understanding protein expression dynamics is crucial for identifying key developmental processes.

Purpose of the Study:

To investigate differential protein expression in the mouse forebrain during postnatal development.
To identify critical biological pathways and molecular players involved in brain maturation.

Main Methods:

Large-scale proteome mapping using two-dimensional difference gel electrophoresis.
Mass spectrometry for protein identification.
Computational Ingenuity Pathway Analysis (IPA).
In situ hybridization for validating transcriptional regulation.

Main Results:

Identified 251 differentially expressed proteins in the mouse forebrain across postnatal stages (P10, P30, Adult).
Most significant molecular changes occurred between P10 and later stages (P30, Adult).
IPA highlighted Semaphorin signaling in neurons and protein ubiquitination as key pathways.
Proteolysis and neurite outgrowth guidance were identified as critical processes.

Conclusions:

Proteolysis and neurite outgrowth guidance are fundamental to early mouse brain maturation.
The identified protein expression profiles and pathways provide a molecular basis for understanding postnatal brain development.