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Smartphone-based simultaneous pH and nitrite colorimetric determination for paper microfluidic devices.

Nuria Lopez-Ruiz¹, Vincenzo F Curto, Miguel M Erenas

¹ECsens-CITIC, Department of Electronics and Computer Technology, ETSIIT, University of Granada , 18071, Granada, Spain.

Analytical Chemistry

|August 27, 2014

Summary

This study presents a smartphone app and paper microfluidic device for measuring pH and nitrite. The app analyzes color changes to provide accurate, low-cost chemical analysis using mobile technology.

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Area of Science:

Analytical Chemistry
Microfluidics
Mobile Sensing

Background:

Traditional chemical analysis methods can be expensive and time-consuming.
There is a growing need for portable and accessible analytical tools.

Purpose of the Study:

To develop a low-cost, mobile-based system for simultaneous pH and nitrite determination.
To validate the use of a smartphone as an analytical instrument.

Main Methods:

An Android application was developed to analyze images of a paper-based microfluidic device.
Immobilized reagents on the device produce color changes in response to sample analytes.
Image processing algorithms extract Hue (H) and Saturation (S) values from the HSV color space for quantification.

Main Results:

The system achieved a pH resolution of 0.04 units and accuracy of 0.09.
Nitrite detection showed a resolution of 0.51% and a limit of detection of 0.52 mg L(-1).
The developed image processing minimized variations due to lighting and device positioning.

Conclusions:

A smartphone can be effectively utilized as a versatile analytical instrument.
The integrated system offers a cost-effective and portable solution for chemical sensing.
This approach demonstrates potential for on-site environmental and food safety monitoring.