Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Maturation of Endosomes01:28

Maturation of Endosomes

5.0K
The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
5.0K
Secretory Phase01:19

Secretory Phase

2.8K
The secretory phase of the menstrual cycle, spanning from day 14 to 28 in a typical 28-day cycle, is a period of significant physiological changes in the female reproductive system. This phase commences immediately after ovulation and is characterized by the preparation of the endometrium for potential embryo implantation.
Following ovulation, the corpus luteum, a temporary endocrine structure, produces progesterone and estrogens. These hormones stimulate the growth and coiling of endometrial...
2.8K
The Nucleolus02:55

The Nucleolus

8.6K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.6K
Folliculogenesis01:20

Folliculogenesis

3.7K
Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...
3.7K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

8.8K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.8K
Bacterial Protein Maturation01:26

Bacterial Protein Maturation

742
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
742

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inherited dentin defects with comorbidities.

Journal of the American Dental Association (1939)·2026
Same author

ODAPH (p.Arg77*) Phenotype and Onset of Ameloblast Pathology During Postsecretory Transition Demonstrated by FIB-SEM Analyses of Odaph<sup>C41*/C41*</sup> Mice.

Calcified tissue international·2026
Same author

Correction: SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion.

eLife·2026
Same author

Hereditary enamel defects with comorbidities.

Journal of the American Dental Association (1939)·2026
Same author

Novel <i>ITGB6</i> Mutations Causing Amelogenesis Imperfecta.

Genes·2026
Same author

Generation and characterization of a murine amelogenesis imperfecta model.

Archives of oral biology·2026

Related Experiment Video

Updated: Apr 25, 2026

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
11:13

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Published on: December 1, 2015

37.9K

Ameloblast transcriptome changes from secretory to maturation stages.

James P Simmer1, Amelia S Richardson, Shih-Kai Wang

  • 1Department of Biologic and Materials Sciences, University of Michigan School of Dentistry , Ann Arbor, MI , USA .

Connective Tissue Research
|August 27, 2014
PubMed
Summary
This summary is machine-generated.

This study identified key genes in dental enamel formation using transcriptome analysis. Protease activated receptor 1 (PAR1) was found to be highly expressed in secretory ameloblasts but not maturation ameloblasts.

Keywords:
AmelogenesisF2RORAI1PAR1STIM1enamel

More Related Videos

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

1.4K
Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
08:12

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants

Published on: March 29, 2018

9.8K

Related Experiment Videos

Last Updated: Apr 25, 2026

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
11:13

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Published on: December 1, 2015

37.9K
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

1.4K
Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
08:12

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants

Published on: March 29, 2018

9.8K

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Amelogenesis, the process of dental enamel formation, involves distinct secretory and maturation stages.
  • Understanding the molecular mechanisms governing these stages is crucial for comprehending enamel development and potential defects.

Purpose of the Study:

  • To identify major molecular components and gene expression changes during the secretory and maturation stages of amelogenesis.
  • To characterize the expression pattern of specific genes, such as F2r (encoding protease activated receptor 1 - PAR1), during amelogenesis.

Main Methods:

  • Laser micro-dissection of ameloblasts from mouse first molars at different developmental stages (secretory and maturation).
  • Next-generation sequencing to perform transcriptome analysis and identify differentially expressed genes.
  • Immunohistochemistry to validate the expression of PAR1 in ameloblasts.

Main Results:

  • Transcriptome analysis revealed 373 genes with higher expression in the secretory stage and 614 genes with higher expression in the maturation stage.
  • Cross-validation with previous studies identified 34 consistently upregulated and 26 downregulated genes.
  • Protease activated receptor 1 (PAR1) showed a 10-fold higher expression in the secretory stage and was localized to secretory ameloblasts, but not maturation ameloblasts.

Conclusions:

  • Transcriptome analysis is a valuable approach for identifying critical genes and proteins involved in dental enamel formation.
  • PAR1 is specifically expressed by ameloblasts during the secretory stage of amelogenesis, suggesting a role in this phase.