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Updated: Apr 25, 2026

Dynamic Light Scattering Analysis for the Determination of the Particle Size of Iron-Carbohydrate Complexes
Published on: July 7, 2023
Effect of particle size distribution on the separation efficiency in liquid chromatography
Krisztián Horváth1, Diána Lukács1, Annamária Sepsey2
1Department of Analytical Chemistry, University of Pannonia, Egyetem utca 10, H-8200 Veszprém, Hungary.
Abstract:
In this work, the influence of the width of particle size distribution (PSD) on chromatographic efficiency is studied. The PSD is described by lognormal distribution. A theoretical framework is developed in order to calculate heights equivalent to a theoretical plate in case of different PSDs. Our calculations demonstrate and verify that wide particle size distributions have significant effect on the separation efficiency of molecules. The differences of fully porous and core-shell phases regarding the influence of width of PSD are presented and discussed. The efficiencies of bimodal phases were also calculated. The results showed that these packings do not have any advantage over unimodal phases.
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