Detection of Mycobacterium tuberculosis in latently infected lungs by immunohistochemistry and confocal microscopy
Selvakumar Subbian1,2, Eliseo Eugenin1, Gilla Kaplan3,1,2
1Public Health Research Institute (PHRI), Rutgers Biomedical and Health Sciences, Rutgers University, Newark, NJ, USA.
Journal of Medical Microbiology
|August 28, 2014
Abstract:
Detection of latent Mycobacterium tuberculosis is a challenge in the diagnosis of asymptomatic, subclinical tuberculosis. We report the development of an immunofluorescence technique to visualize and enumerate M. tuberculosis in latently infected rabbit lungs where no acid-fast-stained organisms were seen and no cultivable bacilli were obtained by the agar-plating method.
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