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Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
Published on: February 6, 2021
Evaluation of bacterial contamination rate of the anterior chamber during phacoemulsification surgery using an
Ibrahim Kocak1, Funda Kocak2, Bahri Teker1
1Nisa Hospital, Fatih cad, Cobancesme mah, Bahcelievler, Istanbul 34196, Turkey.
Aim:
To assess the incidence of anterior chamber bacterial contamination during phacoemulsification surgery using an automated microbial detection system (BacT/Alert).
Methods:
Sixty-nine eyes of 60 patients who had uneventful phacoemulsification surgery, enrolled in this prospective study. No prophylactic topical or systemic antibiotics were used before surgery. After antisepsis with povidone-iodine, two intraoperative anterior chamber aqueous samples were obtained, the first whilst entering anterior chamber, and the second at the end of surgery. BacT/Alert culture system was used to detect bacterial contamination in the aqueous samples.
Results:
Neither aqueous samples obtained at the beginning nor conclusion of the surgery was positive for microorganisms on BacT/Alert culture system. The rate of bacterial contamination during surgery was 0%. None of the eyes developed acute-onset endophthalmitis after surgery.
Conclusion:
In this study, no bacterial contamination of anterior chamber was observed during cataract surgery. This result shows that meticulous surgical preparation and technique can prevent anterior chamber contamination during phacoemulsification cataract surgery.
Insights
This study found no bacterial contamination in the anterior chamber during phacoemulsification cataract surgery. Meticulous surgical techniques effectively prevent microbial contamination, ensuring patient safety.
Area of Science:
- Ophthalmology
- Microbiology
- Surgical Safety
Background:
- Cataract surgery, specifically phacoemulsification, carries a risk of intraocular infection.
- Preventing anterior chamber contamination is crucial for successful surgical outcomes.
Purpose of the Study:
- To evaluate the incidence of bacterial contamination in the anterior chamber during phacoemulsification.
- To assess the efficacy of standard surgical preparation in preventing microbial contamination.
Main Methods:
- Prospective study of 60 patients undergoing phacoemulsification without pre-operative antibiotics.
- Collection of two anterior chamber aqueous samples during surgery (start and end).
- Utilized the automated BacT/Alert culture system for microbial detection.
Main Results:
- Zero percent bacterial contamination was detected in anterior chamber samples.
- No microorganisms were identified at the beginning or end of the surgery.
- No cases of acute-onset endophthalmitis were observed post-operatively.
Conclusions:
- Meticulous surgical preparation and technique effectively prevent anterior chamber contamination during phacoemulsification.
- The study demonstrates a 0% contamination rate, highlighting the safety of the procedure.
- Standard aseptic techniques are sufficient to maintain sterility within the anterior chamber.
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