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Intracellular potentials of toad urinary bladder.

W Nagel1, W Van Driessche

  • 1Physiologisches Institut der Universität München, Federal Republic of Germany.

Pflugers Archiv : European Journal of Physiology
|October 1, 1989
PubMed
Summary

This study investigated sodium transport in toad urinary bladders. Oxytocin was found to stimulate conductive pathways in both apical and basolateral membranes, enhancing sodium transport without altering cell membrane potentials.

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Area of Science:

  • Physiology
  • Membrane Biophysics
  • Renal Physiology

Background:

  • Amphibian urinary bladders are model systems for studying epithelial sodium transport.
  • Understanding the regulation of ion transport is crucial for renal physiology.

Purpose of the Study:

  • To investigate the effects of oxytocin on intracellular potentials and membrane resistance in toad urinary bladders.
  • To elucidate the specific membrane pathways regulated by oxytocin.

Main Methods:

  • Short-circuited urinary bladders of Bufo marinus were impaled with microelectrodes for intracellular potential recordings.
  • Apical sodium entry was inhibited to assess membrane responses.
  • Cellular recordings were obtained before and after oxytocin administration.

Main Results:

  • Intracellular potentials of -80 mV were recorded.
  • Inhibition of apical Na entry led to hyperpolarization and increased apical membrane fractional resistance.
  • Oxytocin administration doubled sodium transport and cellular conductance without changing intracellular potential or apical membrane resistance.

Conclusions:

  • Oxytocin stimulates conductive pathways in both apical and basolateral membranes of the toad urinary bladder.
  • These findings contribute to understanding the complex regulation of epithelial sodium transport.

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