Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

Cholesterol: Significance and Regulation

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Collection-Free Express H<sub>2</sub>O<sub>2</sub> Detection in Breath Aerosol through Its Capillary Condensation Directly onto the Sensor Surface.

ACS sensors·2026

Same author

Potential impact on cholesterol goal achievement and predicted cardiovascular risk by the addition of ezetimibe and bempedoic acid on top of statins: a simulation from the SANTORINI study.

American journal of preventive cardiology·2026

Same author

Significant correlations between blood lipids, cytokines, and C-reactive protein in healthy humans.

Lipids in health and disease·2026

Same author

Schoolboys' experiences of information and communication regarding the human papillomavirus vaccination.

Journal of public health (Oxford, England)·2026

Same author

Breath Aerosol Admittance Orthonormal to Sweat Conductivity as a Reliable 2-D Diagnostic Criterion for Cystic Fibrosis.

Analytical chemistry·2026

Same author

Dissolution Behavior of Uranium Aerosols in Simulated Lung Fluids: Effects of Particle Size.

Health physics·2026

Search research articles

Related Experiment Video

Updated: Apr 25, 2026

Cholesterol Efflux Assay

Cholesterol Efflux Assay

Published on: March 6, 2012

Cholesterol self-powered biosensor.

Alina N Sekretaryova¹, Valerio Beni, Mats Eriksson

¹Department of Physics, Chemistry and Biology, Linköping University , SE-581 83 Linköping, Sweden.

Analytical Chemistry

|August 29, 2014

Summary

This summary is machine-generated.

This study introduces a novel self-powered biosensor for reagentless cholesterol detection in human plasma. This innovative device enhances sensitivity and offers a wide dynamic range for accurate cholesterol monitoring, crucial for heart disease risk assessment.

More Related Videos

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

Published on: January 7, 2019

Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

Published on: January 4, 2016

Related Experiment Videos

Last Updated: Apr 25, 2026

Cholesterol Efflux Assay

Cholesterol Efflux Assay

Published on: March 6, 2012

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

Published on: January 7, 2019

Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

Published on: January 4, 2016

Area of Science:

Biomedical Engineering
Electrochemistry
Biosensor Technology

Background:

Monitoring cholesterol levels is vital for assessing heart disease risk.
Existing methods for cholesterol detection can be complex and require reagents.
There is a need for sensitive, self-powered biosensors for real-time monitoring.

Purpose of the Study:

To develop a novel, single-enzyme, membrane-free, self-powered biosensor for reagentless cholesterol detection.
To investigate the analytical performance of individual electrode systems and the combined self-powered biosensor.
To compare the biosensor's performance with the standard colorimetric cholesterol quantification method.

Main Methods:

Immobilization of cholesterol oxidase in a sol-gel matrix on both cathode and anode.
Electrocatalytic reduction of hydrogen peroxide at the cathode using Prussian blue.
Simultaneous cholesterol oxidation at the anode mediated by cholesterol oxidase.
Fabrication of electrodes on high surface-area carbon cloth.

Main Results:

The self-powered biosensor demonstrated enhanced sensitivity (26.0 mA M(-1) cm(-2)) compared to individual electrodes.
A wide dynamic range for cholesterol detection was achieved, up to 4.1 mM.
Reagentless detection was successfully performed, and results were comparable to standard methods.

Conclusions:

The developed self-powered biosensor offers a promising tool for reagentless cholesterol monitoring.
The single-enzyme, membrane-free design simplifies the detection process.
This technology has potential applications in clinical diagnostics and personalized health management for cardiovascular disease.